Fake Ray Bans
auther:null date:2020-08-20-
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rAAV2 Traffics through both the Late and the Recycling Endosomes in a Dose rAAV2 Traffics through both the Late and the Recycling Endosomes in a Dose-Dependent Fashion Wei Ding1,Fake Ray Bans 2, *, Liang N. Zhang1, 2, *, Charles Yeaman1 and John F. Using imaging and subcellular fractionation techniques, we evaluated the extent of rAAV2 movement through the late (Rab7) and recycling (Rab11) endosomes. Following rAAV2 infection of HeLa cells, immunoisolation of HA or HA endosomes and intracellular colocalization of Cy3-labeled rAAV2 with EGFP or EGFP markers demonstrated dose-dependent trafficking of rAAV2 through the recycling and late endosomal compartments. 100 genomes/cell), rAAV2 predominantly trafficked to the Rab7 compartment. siRNA studies inhibiting either Rab7 or Rab11 demonstrated that reducing Rab11 protein levels more significantly inhibited rAAV2 transduction on a per genome basis compared to inhibition of Rab7. These findings suggest that strategies to shunt viral movement from the late to the recycling endosome may be effective at increasing viral transduction for gene therapy.
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